CRISPRa-mediated transcriptional activation of the SlPR-1 gene in edited tomato plants
07 de febrero de 2023
Les invitamos a leer el artículo: "CRISPRa-mediated transcriptional activation of the SlPR-1 gene in edited tomato plants" en el que colaboró el Dr. Stefan de Folter, Profesor Investigador de Cinvestav UGA-Langebio, así como Leonardo García Murillo estudiante del posgrado de Biotecnología de Plantas Cinvestav y el Dr. Raúl Álvarez, Profesor Investigador de Cinvestav Irapuato.
Autores: Leonardo García-Murillo, Eliana Valencia-Lozano, Nicolás Alberto Priego-Ranero, José Luis Cabrera-Ponce, Fátima Patricia Duarte-Aké, Juan Carlos Vizuet-de-Rueda, Diana Marcela Rivera-Toro, Humberto Herrera-Ubaldo, Stefan de Folter, Raúl Alvarez-Venegas .
Felicitamos al estudiantado y profesorado que contribuyeron en esta investigación por su arduo trabajo.
Abstract: With the continuous deterioration of arable land due to an ever-growing population, improvement of crops and crop protection have a fundamental role in maintaining and increasing crop productivity. Alternatives to the use of pesticides encompass the use of biological control agents, generation of new resistant crop cultivars, the application of plant activator agrochemicals to enhance plant defenses, and the use of gene editing techniques, like the CRISPR-Cas system. Here, we test the hypothesis that epigenome editing, via CRISPR activation (CRISPRa), activate tomato plant defense genes to confer resistance against pathogen attack. We provide evidence that edited tomato plants for the PATHOGENESIS-RELATED GENE 1 gene (SlPR-1) show enhanced disease resistance to Clavibacter michiganensis subsp. michiganensis infection. Resistance was assessed by evaluating disease progression and symptom appearance, pathogen accumulation, and changes in SlPR-1 gene expression at different time points. We determined that CRISPRa-edited plants develop enhanced disease-resistant to the pathogen without altering their agronomic characteristics and, above all, preventing the advancement of disease symptoms, stem canker, and plant death.
Keywords:Tomato, CRISPR/dCas9, PR-1 gene, Epigenome editing, Plant-pathogen interaction.