Emilio Galván- The bidirectional role of GABAA and GABAB receptors during the differentiation process of neural precursor cells of the subventricular zone

20 de marzo del 2024

Invitamos a leer el artículo: "The bidirectional role of GABAA and GABAB receptors during the differentiation process of neural precursor cells of the subventricular zone", realizado por el Dr. Emilio Galván, Investigador de Cinvestav Sede Sur

Autores: Estefanía Gutierrez-Castañeda, Vladimir Alex Martínez-Rojas, Lenin David Ochoa-de la Paz y Emilio J. Galván.

Abstract: The intricate process of neuronal differentiation integrates multiple signals to induce transcriptional, morphological, and electrophysiological changes that reshape the properties of neural precursor cells during their maturation and migration process. An increasing number of neurotransmitters and biomolecules have been identified that serve as molecular signals that trigger and guide this process. In this sense, taurine, a sulfur-containing, non-essential amino acid widely expressed in the mammal brain, modulates the neuronal differentiation process. In this study, we describe the effect of taurine acting via the ionotropic GABA_A receptor and the metabotropic GABA_B receptor on the neuronal differentiation and electrophysiological properties of precursor cells derived from the subventricular zone of the mouse brain. Taurine stimulates the number of neurites and favors the dendritic complexity of the neural precursor cells, accompanied by changes in the somatic input resistance and the strength of inward and outward membranal currents. At the pharmacological level, the blockade of GABA_A receptors inhibits these effects, whereas the stimulation of GABA_B receptors has no positive effects on the taurine-mediated differentiation process. Strikingly, the blockade of the GABA_B receptor with CGP533737 stimulates neurite outgrowth, dendritic complexity, and membranal current kinetics of neural precursor cells. The effects of taurine on the differentiation process involve Ca²⁺ mobilization and the activation of intracellular signaling cascades since chelation of intracellular calcium with BAPTA-AM, and inhibition of the CaMKII, ERK1/2, and Src kinase inhibits the neurite outgrowth of neural precursor cells of the subventricular zone.

Keywords:Taurine, GABA receptors, subventricular cells, neurogenesis, differentiation, morphogenesis.