Examen de Maestría
Arturo Felipe Angeles Padilla
Les invitamos al examen final del ITA. Arturo Felipe Angeles Padilla para obtener el grado de Maestría en Biotecnología de Plantas
🗓️ jueves 21 de noviembre
⏰ 12:00 Seminario público
⏰ 15:00 Lectura del acta
📍 Auditorio Manuel Ortega, Unidad Irapuato
Biotecnología de Plantas | Cinvestav Irapuato
Examen para obtener el grado de Maestría que presenta:
ITA. Arturo Felipe Angeles Padilla
"Transposon mutagenesis in Mycetohabitans, a comprehensive library for functional genomics"
jueves 21 de noviembre de 2024
12:00 horas SEMINARIO PUBLICO
15:00 horas LECTURA DEL ACTA Y AGRADECIMIENTOS
Auditorio de la unidad Irapuato
Manuel Ortega Ortega
DIRECTORA DE TESIS
Dra. Laila Pamela Partida Martínez
COMITÉ TUTORIAL
DRA. Gabriela Olmedo Álvarez
DR. Sean Michael Rovito
RESUMEN
Rhizopus microsporus has received attention due to its pathogenic role in rice seedling blight, attributed to the mycotoxin rhizoxin. Rhizoxin is an antimitotic macrolide which binds to β-tubulin effectively preventing cell division. Recent discoveries have revealed that the true production of rhizoxin is linked to a bacterial endosymbiont, initially classified within the genus Burkholderia, and now recognized as part of the new genus Mycetohabitans.
Rhizopus microsporus relies not only on its bacterial endosymbiont for mycotoxin production but also for its asexual reproduction, which ceases upon the elimination of the symbiont. The restoration of asexual reproduction occurs once the bacteria recolonize the fungal hyphae.
To investigate the genetic basis of asexual reproduction in symbiotic R. microsporus, we aimed to generate a transposon-based mutant library in Mycetohabitans euroasiaticus M7, to identify genes involved in the asexual reproduction of R. microsporus. Unfortunately, we failed in generating this mutant library; whole plasmid sequencing showed the integrity of the pLG99 plasmid, suggesting that this plasmid should be conjugated instead of following the electroporation method. Cross-infection assays proved that symbiotic and cured Rhizopus strains can be infected with various bacterial strains, effectively restoring asexual reproduction.
Additionally, we successfully sequenced a plasmid containing a GFP protein, which was transformed into M. euroasiaticus M7 cells, enabling visualization within fungal spores. We also established a feasible electroporation protocol which works under our laboratory conditions, easing the generation of new mutants. Collectively, these findings contribute to our understanding of the fungal holobiont R. microsporus.